Proteases have evolved to mediate the hydrolysis of peptide bonds but may perform transpeptidation in the presence of a proper nucleophilic molecule that can effectively compete with water to react with the acyl-enzyme intermediate. There have been several examples of protease-mediated transpeptidation. but they are generally inefficient. and little effort has been made to systematica... https://foldlyers.shop/product-category/alarm-clock/